The Technical Committees responsible for this document are CEN/TC 307 "Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis" and CEN/TC 19 "Gaseous and liquid fuels, lubricants and related products of petroleum, synthetic and biological origin". Regarding the German collaboration, the responsible committee is NA 062-06-32-01 UA "Prüfung von FAME" ("Testing of FAME") at the Petroleum, Fuels, Lubricants and Related Products Standardization Committee (FAM) of the Materials Testing Standards Committee (NMP), in collaboration with NA 057-05-05 AA "Gemeinschaftsausschuss für die Analytik von Fetten, Ölen, Fettprodukten, verwandten Stoffen und Rohstoffen" ("Joint Committee for the analysis of fats, oil, fat products, related substances and raw materials") of the Food and Agricultural Products Standards Committee (NAL). This document specifies a procedure for the determination of the ester content in fatty acid methyl esters (FAME) intended for incorporation into diesel oil. It also allows determining the linolenic acid methyl ester content. It allows verifying that the ester content of FAME is greater than 90 % (m/m) and that the linolenic acid content is between 1 % (m/m) and 15 % (m/m). This method is suitable for FAME which contains methyl esters between C6 and C24.