DIN EN 16006 is applicable to the determination of Fumonisin B1 (FB1) and B2 (FB2) in compound animal feed at levels starting from 3 mg/kg up to 16 mg/kg. FB1 and FB2 are extracted from the test material with a solution of 50 % methanol in phosphate-buffered saline (PBS). The extract is then diluted with PBS and cleaned up using immunoaffinity columns (IAC). FB1 and FB2 are eluted from the IAC using methanol and then water. After volume adjustment, the eluate is directly injected into the HPLC and FB1 and FB2 are detected by their fluorescence after either pre- or post column derivatisation.
The European Standard has been prepared by CEN/TC 327 "Animal feeding stuffs - Methods of sampling and analysis", the secretariat of which is held by NEN (Netherlands). The collaboration of DIN for the field of responsibility of CEN/TC 327 is performed through national Mirror Committee, Working Committee NA 057-03-03 AA "Futtermittel" ("Animal feeding stuffs") at NAL.