DIN EN ISO 15753:2016-11

This International Standard describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: - a general method; and a method specific for coconut oil and vegetable oils with short-chain fatty acids.
These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this International Standard.
The polycyclic aromatic hydrocarbons are extracted with an acetonitrile/acetone mixture followed by purification on C18 reversed-phase and then Florisil bonded-phase cartridges. Determination of the content of the individual polycyclic aromatic hydrocarbons after separation is achieved by means of high-pressure liquid chromatography (HPLC) and by measuring the fluorescence at various excitation and emission wavelengths.
The International Standard has been prepared by ISO/TC 34/SC 11 "Animal and vegetable fats and oils" (secretariat: BSI, United Kingdom) of Technical Committee ISO/TC 34 "Food products" (secretariat: AFNOR, France). CEN/TC 307 "Oilseeds, vegetable and animal fats and oils and their by-products - Sampling and test methods" (secretariat: AFNOR, France) is responsible for the European Standard. On the national level, "Gemeinschaftsausschuss für die Analytik von Fetten, Ölen, Fettprodukten, verwandten Stoffen und Rohstoffen" (GA Fett) ("Joint Committee for the analysis of fats, oil, fat products, related substances and raw materials") of the German Society for Fat Science (DGF) and DIN Standards Committee Food and Agricultural Products (NAL) is responsible for the standard.