DIN EN ISO 12966-4:2015-11

This part of ISO 12966 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters. This International Standard is Part 4 of a series of four International Standards for the manufacture and determination of fatty acid methyl esters by gas chromatography in animal and vegetable fats and oils. ISO 12966 (all parts) is suitable for crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources. This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966. This part of ISO 12966 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils. Part 1 of ISO 12966 is a guideline on modern gas chromatography of fatty acid methyl esters whereas ISO 12966-2 and ISO 12966-3 cover the manufacture of fatty acid methyl esters using various methods. Part 4 of ISO 12966 contains the conditions for analysis of fatty acid methyl esters (FAME) by capillary gas chromatography. The document contains without any modifications the International Standard ISO 12966-4:2015 which has been prepared by ISO/TC 34 "Food products" (secretariat: AFNOR, France), Subcommittee SC 11 "Animal and vegetable fats and oils" (secretariat: BSI, United Kingdom). CEN/TC 307 "Oilseeds, vegetable and animal fats and oils and their by-products - Sampling and test methods" (secretariat: AFNOR, France) is responsible for the European Standard. On the national level, NA 057-05-05 AA "Gemeinschaftsausschuss für die Analytik von Fetten, Ölen, Fettprodukten, verwandten Stoffen und Rohstoffen" (GA Fett) ("Joint Committee for the analysis of fats, oil, fat products, related substances and raw materials") of the German Society for Fat Science (DGF) and NAL are responsible for the standard.